Determination of Absolute Purities of Hygroscopic Substances by Quantitative NMR Analysis for the Standardization of Quantitative Reagents in the Japanese Pharmacopoeia (Part 2)

As a new absolute quantitation method for low-molecular compounds, quantitative NMR (qNMR) has emerged. In the Japanese Pharmacopoeia (JP), 15 compounds evaluated by qNMR are listed as reagents used HPLC reference standards in assay of crude drug section JP. previous study, we revealed that humidity affects purity values hygroscopic and (i) control before during weighing is important reproducible preparation (ii) indication amount (not value), which not affected water content, products determined qNMR. this typical optimal conditions affect determination ginsenoside Rb1 (GRB1), saikosaponin (SSA), barbaloin (BB) (i.e., reagents) were examined. First, effect on commercial GRB1, with value qNMR, was The results showed importance afore-mentioned. SSA, relatively unstable dissolved state, suggested standardization specific time provides practical approach products. regard to BB, its time, only weighing, enough determination.